Identification and Characterization of a Cold-Active Phthalate Esters Hydrolase by Screening a Metagenomic Library Derived from Biofilms of a Wastewater Treatment Plant

Novel Cold-Adapted Esterase MHlip from an Antarctic Soil Metagenome

An Antarctic soil metagenomic library was screened for lipolytic enzymes and allowed for the isolation of a new cytosolic esterase from the a/b hydrolase family 6, named MHlip. This enzyme is related to hypothetical genes coding esterases, aryl-esterases and peroxydases, among others. MHlip was produced, purified and its activity was determined. The substrate profile of MHlip reveals a high spe...

Functional screening of a metagenomic library of seaweed - associated 1 microbiota : identification and characterization of a halotolerant , cold - 2 active marine endo - ß - 1 , 4 - endoglucanase 3 4

Evaluation of the Phthalate Esters in South Indian Women with Endometriosis

Background To evaluate the possible association between phthalate esters (PEs) and the occurrence of endometriosis. Blood samples were collected from 99 infertile women with endometriosis (study group); 135 age-matched women without endometriosis (control group) but with infertility related to tubal defects, fibroids, polycystic ovaries, idiopathic infertility and pelvic inflammatory diseases d...

Identification and characterization of a halotolerant, cold-active marine endo-β-1,4-glucanase by using functional metagenomics of seaweed-associated microbiota.

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection perio...

A concern on phthalate pollution of herbal extracts/medicines and detection methods

Esters of phthalates, mainly applied as plasticizer, cause several human health and environment    hazards. Phthalates are widely used in pharmaceutical products, cosmetics, as well as other plastic commercial products, and can penetrate in foods, water dusts, and air leading to ingestion and inhalation exposure followed by skin absorption for human. These compounds cause serious adverse effect...